Automated Calorimetry Facility

Isothermal Titration Calorimetry

Isothermal titration calorimetry (ITC) is a technique that measures the heat absorbed or released during a binding event. Integrating the raw data leads to the calculation of the enthalpy of binding. A curve is fit with an appropriate model using the data plot of the enthalpy and ligand-to-macromolecule mole ratio. From this curve the thermodynamic parameters, namely the stoichiometry, dissociation constant, and binding entropy, are obtained.

Instrumentation

MicroCal Auto-iTC200

The Auto-iTC200 combines the performance of the most sensitive isothermal titration calorimeter available with full automation.