Isothermal titration calorimetry (ITC) is the gold standard analytical techniques for in-depth characterization of molecular binding events.
Uses
• Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules
• Enzyme kinetics
• Assessment of molecular structure changes on binding mechanisms
Specifications
• Operating temperature: 2°C to 80°C
• Sample storage temperature: 4°C to ambient
• Response time: 10 seconds
• Cell design: 200µl coin-shaped
• Titration syringe: 40µl
• Usable syringe volume: 38µl
• Smallest injection size: 0.1µl
• Stirring speed: 500-1,500 rpm
Details
MicroCal Auto-ITC200 is fully automated. Users can set up samples in 96 well plate in a controlled temperature autosampler. Automated cleaning can also be programmed to reduce contamination between runs.
Acknowledgement
Contributions made by data from this instrument may be acknowledged in papers with language similar to the example below:
We would like to acknowledge Dr. Neela Yennawar and Julia Fecko from the Pennsylvania State University Automated Biological Calorimetry facility for use of Auto ITC200 – NSF-MRI award DBI-0922974 (Bevilacqua et al 2009).