The Huck Institutes of the Life Sciences

Transgenic mouse production

Production of transgenic mice by pronuclear injection

What you will need

Investigators should have:

  • A PSU IACUC approved protocol so that any transgenic progeny can be transferred to your protocols at weaning
  • Evidence of a screening assay, either PCR or Southern, for your proposed transgene
  • Consulted with the Animal Resources Program to acquire mouse space in one of the PSU campus facilities
  • Consulted with us to establish protocols for isolation of and preparation of DNA constructs