The Huck Institutes of the Life Sciences

Protocols

Standard preparation procedures for methods used at the Transgenic Mouse Facility

Isolating DNA from mouse tail clips

Procedure used by the Transgenic Mouse Facility to isolate DNA from mouse tail clips

 

Preparation of DNA construct for electroporation in embryonic stem (ES) cells

Procecure used by the Transgenic Mouse Facility to prepare DNA constructs for electroporation in embryonic stem (ES) cells

 

Preparation of transgene for microinjection: sucrose gradient method

Procedure used by the Transgenic Mouse Facility to prepare transgenes for microinjection (sucrose gradient method)

 

Preparation of transgene for pronuclear microinjection

Procedure used by the Transgenic Mouse Facility to prepare transgenes for pronuclear microinjection