The Transgenic Mouse Facility is a semi-barrier facility located in Life Sciences Building.
We have animal space in Life Sciences Building, which is used for mouse microinjection procedures and holding of potential transgenic mice until weaning.
Mice are housed in ventilated micro-isolator caging with access limited to designated personnel.
Our microinjection suite is equipped with an Olympus IX-70 microscope equipped with Hoffman Contrast Optics.
The scope is attached to Eppendorf Transferman 4r micromanipulators and Celltram microinjectors, and is fixed with an Eppendorf Femtojet 4i for pronuclear injections.
The suite also contains two surgical scopes for embryo collection and transplantation, a De Fonbrune microforge for fashioning micropipettes, a Sutter P-97 micropipette puller, 2 incubators and 2 refrigerator/freezers.
Accreditation and oversight
This facility is accredited with the Association for the Assessment and Accreditation of Laboratory Animal Care, International (AAALAC), and is overseen by a federally mandated Institutional Animal Care and Use Committee (IACUC) established through Penn State's Office for Research Protections.