The Huck Institutes of the Life Sciences


Services provided by the Microscopy and Cytometry Facility at University Park

The Microscopy and Cytometry Facility provides a wide variety of services including microscopy, flow cytometry, histology, sample preparation, training, and consultation.


Electron microscopy services

  • Conventional transmission electron microscopy (TEM) (ultrastructure analysis)
  • Scanning electron microscopy (SEM) (secondary and backscattered electron images)
  • Sample processing and embedding
  • Cryo-SEM
  • Cryo-TEM
  • Ultramicrotomy and cryo-ultramicrotomy
  • Immunogold labeling
  • Negative staining
  • Electron tomography
  • Cryofixation and freeze-substitution

View electron microscopes


    Flow cytometry services

    • Flow cytometry
    • Cell sorting (FACS)
    • Imaging cytometry

    View flow cytometers


      Fluoresence and confocal microscopy services

      • Brightfield
      • Darkfield
      • Differential interference contrast (DIC)
      • Polarized microscopy
      • Epifluorescence
      • Confocal
      • Live cell imaging

      View fluorescence and confocal microscopes


        Histology services

        • Paraffin processing
        • Paraffin microtomy and frozen sectioning
        • Automated H&E staining
        • Histochemistry 

        View accessory equipment


          Laser-capture microdissection services

          • Microdissection of cells and tissues under a microscopic objective lens

          View laser-capture microdissection equipment


          Training and consultation

          • Training in sample preparation, use of equipment, and interpretation of results
          • Information to help you in grant and paper preparation, for example literature searches, reference materials, preliminary data collection and experimental design
          • Access to Current Protocols in Cytometry, the Buffalo confocal, Microscopy society and Purdue cytometry newsnets for help with difficult problems
          • Workshops and facility tours
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