The Huck Institutes of the Life Sciences

Confocal microscope

Model: Olympus Fluoview 300

A basic confocal laser scanning microscope requiring manual filter and laser set-ups, and capable of highly sensitive and sophisticated multi-color imaging

 

Specifications

  • Blue, red and green lasers for multi-color imaging
  • X, Y, Z scanning for 3-D reconstruction

 

Details

Microscope:
Olympus IX70 inverted microscope with fluorescence burner
Objectives:
Uplan4X, UplanFL 10X/0.30, planFL 20X/0.50, UplanFL 40X/0.75, PlanApo 60X/1.4oil, Plan Apo 100X/1.3
Lasers:
Three single-line lasers with individual shutters that are software-controlled for sequential acquisition.
  • Blue argon (488nm, 10mW)
  • Green HeNe (543nm, 10mW)
  • Red HeNe (633nm, 10mW)
Scanning modes:
  • 1-dimensional (spot-scan)
  • 2-dimensional (X-Y vector, X-Z, or time)
  • 3-dimensional (X-Y-Z, or X-Y-time)
  • 4 dimensional (X-Y-Z-time)
Transmitted light:
  • External halogen light source connected to microscope via fiber cables, external PMT for light collection.
  • DIC optics are available for all objectives.
Resolution:
Confocal LSM improves the resolution of images by recording fluorescence or reflected light when a laser is focused at set focal planes in tissues and cells, rejecting all other light coming from planes above or below the one of interest. This improves resolution of sub-micron structures within fixed and live cells and tissues. A series of images can be recorded axially and either analyzed separately or incorporated into a composite image.
Software:
Fluoview: captures files as MultiTIFF files (*.oib) and displays as TIFF files (*.tiff)
Output:
  • Files will be transferred to an off-line computer.
  • Users will transfer data via FTP or download it to a writeable CD-ROM.
  • Display TIFF images can be printed directly using graphics software (such as Photoshop or PowerPoint).
  • MultiTIFF images can be reanalyzed using the Fluoview software or ImageJ.

 

Equipment Location

Microscopy and Cytometry Facility

 
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