The Huck Institutes of the Life Sciences

Beckman Coulter FC500

A dual-laser flow cytometer with detection of up to 5 colors of fluorescence; users run samples with minimal help after initial training.

 

Build a standard panel

See alternate configurations

 

Details

  • Particle size sensitivity and range:  Resolves 0.5um particles from background with a maximum detection of up to 40um diameter particle.
  • Lasers:  2 (488nm, 633nm)
  • Simultaneous colors:  5
  • Instrument can accept samples from:  12mm x 75mm tubes only (provided by MCF)
    • Instrument can accept tubes manually OR automatically run 32 in a row
  • Researchers can use independently following 2-hour hands-on training session
  • BSL-1 materials only (must be fixed if BSL-2 or greater)
  • Software:  CXP software controls the instrument.
  • Listmode data files can be collected and reanalyzed in offline flow cytometry software packages such as FCS Express or FlowJo.

 

Operation Manual

Policies

 

Equipment Location

W-124A Millennium Science Complex

Microscopy and Cytometry Facility