The Huck Institutes of the Life Sciences

Services and Instrumentation

Services provided and research instruments hosted by the Metabolomics Core Facility at University Park

The Facility has two hybrid Quadrupole Time-of-Flight (Q-TOF) tandem mass spectrometers, an AB SCIEX 5600 Triple TOF with a Shimadzu Prominence UFLC, and a Waters Synapt G2-S with a Waters Acquity UPLC.

 

These instruments are used for liquid chromatography-mass spectrometry (LC-MS/MS) based global profiling and identification of the metabolites present in biofluids and tissue extracts.

 

A Thermo Scientific EXACTIVE PLUS high resolution mass spectrometer is also used for LC-MS based global profiling of metabolites, but with a targeted data analysis.

 

Two triple quadrupole mass spectrometers (Waters Xevo TQ-S, and Waters Xevo TQ-D, both with Waters Acquity UPLCs) are used for LC-MS/MS based targeted analysis and for validation and accurate quantitation of compounds identified in the global profiling approach.

 

An Agilent gas chromatography-mass spectrometry (GC-MS) system is available for the analysis of compounds not amenable to liquid-chromatography-mass spectrometry.

 
AB SCIEX 5600 TripleTOF
Uniquely integrates comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform, combining the highest sensitivity detection, high-resolution with at least 5X better acquisition speed, and stable ~1ppm mass accuracy over days of acquisition
Waters SYNAPT G2-S
Featuring Stepwave™ technology and the high resolution QuanTof™ mass analyzer, the SYNAPT G2-S MS provides the highest sensitivity and selectivity for very challenging qualitative and quantitative applications. The Lockspray system provides routine sub-ppm mass accuracy.
Waters Xevo TQ-S
A benchtop QTof mass spectrometer with StepWave and QuanTof technologies for scientists who need to confidently identify, quantify and confirm unknown compounds in complex samples — the Xevo G2-S QTof features IntelliStart technology, an intuitive interface that automates routine tasks and ensures reproducible UPLC/MS data of the highest quality.