Model #: Illumina MiSeq
This instrument is essentially a small version of the HiSeq 2500.
- 15 million single reads or 30 million paired reads per run
- Fragment sequencing up to 250 nt
- Paired end sequencing up to 250 x 250 nt
- Sample multiplexing available
The sequencing chemistry is similar to the HiSeq, and all libraries compatible with the HiSeq can be sequenced with the MiSeq.
The MiSeq produces a modest number of reads; therefore, it is best used for pilot projects or for applications requiring small numbers of reads such as de novo sequencing or resequencing of small genomes, amplicon sequencing, and miRNA/small RNA sequencing.
Sample multiplexing is available.
Data is produced in the FASTQ format.
This instrument is used by Genomics Core Facility staff in conjunction with provided next-generation sequencing services.