The Huck Institutes of the Life Sciences

Announcing major equipment acquisitions at the Huck Institutes

The Huck Institutes of the Life Sciences, in conjunction with the Office of the Vice President for Research and the Office of the Provost, are pleased to announce several major equipment acquisitions that will further strengthen the research capabilities of their Core Facilities at University Park.

These items will be appearing and installed over the coming months.

Please check the websites of the individual facilities for updates.

The acquisitions include the following:

Zeiss Sigma Variable Pressure – Field Emission Scanning Electron Microscope with Gatan 3View accessory

As a leading scanning electron microscopy platform, the Sigma VP FE-SEM offers the greatest flexibility in the imaging and analysis of both conducting and non-conducting specimens; the Gatan 3View2 attachment provides researchers the ability to obtain in situ 3D data by operating a high precision ultramicrotome within a field emission gun scanning electron microscope. The 3View2 is an automated sectioning and image capture system that lends itself to unattended operation resulting in a stack of aligned images which can be viewed in sequence or further processed using 3D reconstruction and measurement software. This SEM replaces the 22 year old Jeol JSM5400.

More information is available on the Zeiss and Gatan websites.

Contact

Greg Ning, Microscopy and Cytometry Facility


Jasco J-1500 High Performance Circular Dichroism Spectrometer

The new JASCO J-1500 is designed to meet the most demanding of CD applications. The performance level is unparalleled and the layout is suited for adaptation to a variety of different sampling methods. CD, LD and Absorbance are provided as standard, wavelength range 163-950nm, Temperature controlled -40 – 130oC. The new Simultaneous Multi-Probe (SMP) acquisition system allows you to unleash the power only dreamed of by earlier systems.

More information is available on the Jasco website.

Contact

Neela Yennawar, X-Ray Crystallography and Automated Biological Calorimetry Facilities


EMD Millipore Flowsight Imaging Cytometer

The FlowSight operates like a conventional flow cytometer but also provides imagery of every cell. Powerful and intuitive analysis software seamlessly links quantitative data to imagery. The FlowSight stands apart from other flow cytometers by producing up to 12 images of each and every cell. The unique image collection system simultaneously produces a side scatter (darkfield) image, one or two transmitted light (brightfield) images, and up to 10 fluorescence images.

More information is available on the Amnis website.

Contact

Greg Ning, Microscopy and Cytometry Facility


Beckman Coulter 355nm and 405nm laser and EQ cell sorting upgrades to Astrios cell sorter

This two laser upgrade will add functionality to the recently acquired BC Astrios cell sorter that can perform BSL 1, 2 and 2+ sorts. The 355nm UV laser will allow for sorting populations that lack fluorophores, also Indo-1 and DAPI. The 405nm laser allows for sorting on the popular fluorophores: DAPI, Hoechst, Alexa Fluor 405 and BFP. The EQ cell sorting technology provides the ability to simultaneously observe sub-micron particles and macro cells in the same dynamic range. Thus, the ability to sort chromosomes becomes a possibility, allowing for the generation of chromosome specific DNA libraries, probes for reverse painting, array painting and related techniques.

Contact

Greg Ning, Microscopy and Cytometry Facility


Nanostring nCounter Analysis System

The nCounter® Analysis System is an automated, multi-application, digital detection and counting system which directly profiles up to 800 targets simultaneously from a single sample using a novel barcoding technology. The digital detection of target molecules and high levels of multiplexing eliminate the compromise between data quality and data quantity, producing gold-standard sensitivity and reproducibility for studies of hundreds of targets. The system uses molecular “barcodes” and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.

Contact

Deb Grove, Genomics Core Facility

More information is available on the Nanostring website.


Formulatrix Rock Imager 182

The Rock Imager is an automated imaging system for protein crystallization. Formulatrix' UV imaging technology uses the fluorescence of tryptophan under ultraviolet light to help determine whether a particular crystal is protein or not and aids in finding hidden crystals not visible with bright-field imaging. This robust, easy-to-use robot incubates and captures superior quality images of up to 182 microplates on a user-defined schedule.

More information is available on the Formulatrix website.

Contact

Neela Yennawar, X-Ray Crystallography Facility


AKTA Pure 150M Fast Protein Liquid Chromatograph (FPLC)

The Fast Protein Liquid Chromatography system (formerly Amersham Biosciences) is a new bio-compatible high performance liquid chromatography system for fast and easy purification of proteins. The system includes pumps (up to 150ml/min), gradient mixer, three wavelength detector, conductivity detector, fraction collector (up to 175 fractions of 3-50ml), column switching between up to 5 columns.

More information is available on the GE Healthcare Life Sciences website.

Contact

Mark Signs, Shared Fermentation Facility


Bruker UltrafleXtreme MALDI-TOF/TOF

To expand our services into the areas of high-throughput proteomics and metabolomics, MS-based molecular imaging, high-throughput microorganism identification, and bioengineering applications and those areas of MS and MS2 analysis where MALDI is either the only possible method of ionization or complements ESI in a unique way. The low analyte requirements, the amount of analyte entering the mass analyzer along with ease of use make the UltrafleXtreme a perfect hands-on multi-user instrument that also serves our synthetic chemistry community.

More information is available on the Bruker website.

Contact

Tania Laremore, Proteomics and Mass Spectrometry Core Facility


Upgrade to a 7T Bruker BioSpec 70/30 Magnetic Resonance Imaging system

This upgrade includes the Bruker Avance Console upgrade (AVIII HD) for the existing Agilent 7T horizontal bore instrument located within the High Field MRI Facility in the basement of Chandlee Laboratory. This upgrade was brought about in part by the recent announcement that Agilent Technologies will no longer support preclinical MRI systems. Areas including: three dimensional anatomical MRI, (resting state) fMRI, and dynamic contrast enhanced MRI, DTI and tractography, arterial spin labeling, MR spectroscopy and spectroscopic imaging, MR angiography and flow MRI, susceptibility weighted MRI, zero TE MRI, and cardiac imaging. Animal behaviors, optogenetics, and animal models of brain disorders and other disease all stand to benefit from this upgrade.

More information is available on the Bruker website.

Contact

Thomas Neuberger, High Field MRI Facility


Leica EM CPD300 Critical Point Dryer

Critical point drying is an efficient method for drying delicate samples. It preserves the surface structure of a specimen which could otherwise be damaged due to surface tension when changing from the liquid to gaseous state. This table-top critical point dryer gently dries biological specimens such as pollen, tissue, plants, insects etc.

More information is available on the Leica website.

Contact

Greg Ning, Microscopy and Cytometry Facility


Gatan 914 Cryo Tomography Holder for FEI BioTWIN TEM

The 914 High Tilt Liquid Nitrogen Cryo Transfer Tomography Holder is designed for low temperature transfer and subsequent tomographic recording of electron beam sensitive, frozen hydrated specimens in the TEM. The low profile tip design of the 914 provides a large field of view at high tilt angles to facilitate frozen hydrated tomographic data collection.

More information is available on the Gatan website.

Contact

Greg Ning, Microscopy and Cytometry Facility