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Instrumentation

Equipment and software used in the Proteomics and Mass Spectrometry Core Facility at University Park.

Mass Spectrometers

  • Waters QTOF Premier (capLC-nanoESI-MS/MS)
  • Waters QTOF Micro (capLC-nanoESI-MS/MS)
  • Waters LCT Premier (LC-API-MS)
  • Applied Biosystems 3200QTRAP (LC-API-MS/MS)
  • Applied Biosystems API 150EX (LC-ESI-MS)
  • Waters GCT (GC-EI/CI-MS)
  • Waters M@LDI L/R (MALDI-MS)

HPLC

  • Agilent 1100 Series HPLC with UV-photo diode array detection and microfraction collector

Sample preparation

  • Multiphor II electrophoresis unit (Amersham Biosciences)
  • Ettan DALTsix electrophoresis unit (Amersham Biosciences)
  • Protean IEF cell (Bio-Rad Labs)
  • Criterion Dedeca cell for SDS-PAGE (Bio-Rad Labs)
  • Criterion electrophoresis cell (Bio-Rad Labs)
  • Criterion protein blotter (Bio-Rad Labs)
  • GS-800 calibrated densitometer (Bio-Rad Labs)
  • Molecular imager FX Pro Plus laser scanner (Bio-Rad Labs)
  • Proteome Works spot cutter with fluorescent enclosure (Bio-Rad Labs)
  • PDQuest server (Bio-Rad Labs)
  • SpectraMAX 190 plate reader (Molecular Devices Corp)
  • Speed Vac (Savant)

Software

  • MASCOT (Matrix Science)
  • PDQuest software (multi-user network license) for 2-D electrophoresis (Bio-Rad Labs)
  • Quantity One software for SDS-PAGE (Bio-Rad Labs)