Instrumentation
Equipment and software used in the Proteomics and Mass Spectrometry Core Facility at University Park.
Mass Spectrometers
- Waters QTOF Premier (capLC-nanoESI-MS/MS)
- Waters QTOF Micro (capLC-nanoESI-MS/MS)
- Waters LCT Premier (LC-API-MS)
- Applied Biosystems 3200QTRAP (LC-API-MS/MS)
- Applied Biosystems API 150EX (LC-ESI-MS)
- Waters GCT (GC-EI/CI-MS)
- Waters M@LDI L/R (MALDI-MS)
HPLC
- Agilent 1100 Series HPLC with UV-photo diode array detection and microfraction collector
Sample preparation
- Multiphor II electrophoresis unit (Amersham Biosciences)
- Ettan DALTsix electrophoresis unit (Amersham Biosciences)
- Protean IEF cell (Bio-Rad Labs)
- Criterion Dedeca cell for SDS-PAGE (Bio-Rad Labs)
- Criterion electrophoresis cell (Bio-Rad Labs)
- Criterion protein blotter (Bio-Rad Labs)
- GS-800 calibrated densitometer (Bio-Rad Labs)
- Molecular imager FX Pro Plus laser scanner (Bio-Rad Labs)
- Proteome Works spot cutter with fluorescent enclosure (Bio-Rad Labs)
- PDQuest server (Bio-Rad Labs)
- SpectraMAX 190 plate reader (Molecular Devices Corp)
- Speed Vac (Savant)
Software
- MASCOT (Matrix Science)
- PDQuest software (multi-user network license) for 2-D electrophoresis (Bio-Rad Labs)
- Quantity One software for SDS-PAGE (Bio-Rad Labs)