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Proteomics and Mass Spectrometry Core Facility, University Park
Offers nominal and accurate mass spectrometry analysis of synthetic compounds, polymers and biomolecules (such as proteins, peptides, oligosaccharides and oligonucleotides or their conjugates). Provides identification and quantitation of metabolites by LC-MS/MS and GC-MS, protein sample preparation for mass spectrometry analysis and subsequent identification by MALDI-TOF MS (peptide mass fingerprinting) or nanoflow LC ESI tandem mass spectrometry (nano LC-MS/MS). Assists in data interpretation.
Molecular mass determination of small molecules: routine services
- Confirmation of reaction products
- Nominal mass and accurate mass measurements (<10ppm)
- Analysis of synthetic compounds, polymers and biomolecules (intact proteins (<60KDa), peptides, lipids (<1KDa), and oligonucleotides)
- Ionization methods: ESI (+/-), APCI (+/-), APPI (+/-), MALDI (+), EI (+), CI (+)
- Mixture analysis by LC-MS and GC-MS
Proteomics: routine services
- 2-D gel electrophoresis
- 2-D differential gel electrophoresis (DIGE)
- Image acquisition by densitometer and laser scanner
- Image analysis using PDQuest
- Sample preparation for mass spectrometry analysis (spot-picking, in-gel digestion, in-solution digestion, desalting)
- Protein identification by peptide mass fingerprinting (MALDI-MS) and tandem mass spectrometry (LC-MS/MS): Mascot search against standard databanks (Swissprot, NCBInr)
Non-routine services
- Custom sample preparation
- Liquid/Gas Chromatography-Mass Spectrometry method development
- Quantitation
- Post-translational modification analysis
- De novo peptide sequencing
- 2-D gel electrophoresis workshop
Please make an appointment with us to discuss non-routine research needs, collaboration, and/or consultation.