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Proteomics and Mass Spectrometry Core Facility, University Park

Offers nominal and accurate mass spectrometry analysis of synthetic compounds, polymers and biomolecules (such as proteins, peptides, oligosaccharides and oligonucleotides or their conjugates). Provides identification and quantitation of metabolites by LC-MS/MS and GC-MS, protein sample preparation for mass spectrometry analysis and subsequent identification by MALDI-TOF MS (peptide mass fingerprinting) or nanoflow LC ESI tandem mass spectrometry (nano LC-MS/MS). Assists in data interpretation.

Molecular mass determination of small molecules: routine services

  • Confirmation of reaction products
  • Nominal mass and accurate mass measurements (<10ppm)
  • Analysis of synthetic compounds, polymers and biomolecules (intact proteins (<60KDa), peptides, lipids (<1KDa), and oligonucleotides)
  • Ionization methods: ESI (+/-), APCI (+/-), APPI (+/-), MALDI (+), EI (+), CI (+)
  • Mixture analysis by LC-MS and GC-MS

Proteomics: routine services

  • 2-D gel electrophoresis
  • 2-D differential gel electrophoresis (DIGE)
  • Image acquisition by densitometer and laser scanner
  • Image analysis using PDQuest
  • Sample preparation for mass spectrometry analysis (spot-picking, in-gel digestion, in-solution digestion, desalting)
  • Protein identification by peptide mass fingerprinting (MALDI-MS) and tandem mass spectrometry (LC-MS/MS): Mascot search against standard databanks (Swissprot, NCBInr)

Non-routine services

  • Custom sample preparation
  • Liquid/Gas Chromatography-Mass Spectrometry method development
  • Quantitation
  • Post-translational modification analysis
  • De novo peptide sequencing
  • 2-D gel electrophoresis workshop

Please make an appointment with us to discuss non-routine research needs, collaboration, and/or consultation.

Tours

Want to tour a facility at University Park, or arrange a tour for someone else?

The Huck Institutes’ facility directors are glad to assist with scheduled tours for potential faculty hires and student recruitment. However, due to the heavy workloads in our facilities, we must request that the tours be set up in advance. Most of our facility directors cannot accommodate “drop in” tours since they are working on time sensitive research projects for faculty.

Procedure for setting up facility tours

Contact the facility director to schedule the tour. We request a one week notice prior to the potential visit (earlier if possible). Or, if you prefer, you can contact Megan Matthews to make your arrangements.