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Methyl sequencing

DNA methylation patterns can affect phenotype. Patterns can change in response to spatial, temporal and environmental cues. A method to determine sites of methylation is now available for use with the DNA Sequencer 3730XL.

The basic protocol involves converting all non-methylated Cs to Ts using bisulfite. The resulting gDNA sample will have no Cs present in the sequencing data. Consequently, we can identify methylated cytokines by comparing bisulfite-treated sequences with equivalent untreated sequences.