Services

The Microscopy and Cytometry Facility provides a wide variety of services, including microscopy, flow cytometry, histology, sample preparation, training and consultation.

Electron microscopy services

  • Conventional transmission electron microscopy (TEM) (ultrastructure analysis)
  • Scanning electron microscopy (SEM) (secondary and backscattered electron images)
  • Sample processing and embedding
  • Cryo-SEM
  • Ultramicrotomy and cryo-ultramicrotomy
  • Immunogold labeling
  • Negative staining
  • Cryofixation and freeze-substitution

Flow cytometry services

  • Flow cytometry
  • Cell sorting (FACS)

Fluoresence and confocal microscopy services

  • Brightfield
  • Darkfield
  • Differential interference contrast (DIC)
  • Polarized microscopy
  • Epifluorescence
  • Confocal

Histology services

  • Paraffin processing
  • Paraffin microtomy and frozen sectioning
  • Automated H&E staining

Training and consultation

  • Training in sample preparation, use of equipment, and interpretation of results
  • Information to help you in grant and paper preparation, for example literature searches, reference materials, preliminary data collection and experimental design
  • Access to Current Protocols in Cytometry, the Buffalo confocal, Microscopy society and Purdue cytometry newsnets for help with difficult problems
  • Workshops and facility tours