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Services
Services performed by the Genomics Core Facility, University Park
Genetic analysis services
DNA sequencing - Sanger
- Regular sequencing: standard plasmid and PCR products as well as large DNA services (including genomic)
- High-throughput sequencing (96-well plate submissions). Please inquire as to the specifications for submitting plates before submitting them.
All sequencing requests must be made using the LIMS system at http://tanager.huck.psu.edu. If you bring us DNA of less than 10kilobases by 9am, your sequence should typically be ready to download from http://tanager.huck.psu.edu by 5pm (however, 96-well plates are not guaranteed to be set up the same day we receive them, so results can take slightly longer).
Next generation DNA sequencing - SOLiD
- Targeted resequencing
- Whole genome resequencing
- Chromatin immunoprecipitation (CHIP) analysis
Genotyping
Genemapping and SNP determination.
Quantitative Real-Time PCR
Quantification of DNA and RNA.
DNA extraction and storage
DNA synthesis
We can prepare oligos with lengths of 10 to 150 bases. Crude oligos are usually available within 24-48 hours (see the "Oligo" Announcements section at http://tanager.huck.psu.edu for latest estimates of turnaround times).
Other techniques
Please contact us if you are interested in any other techniques (e.g. rolling circle protocol, genomic sequencing). We will try to provide new services if requested.
Expression analysis services
Next generation sequencing - SOLiD
- Transcriptome analysis
- Expression analysis
- Small RNA analysis
Microarray
- RNA sample labeling, hybridization, scanning, and data acquisition for NimbleGen microarrays.
- RNA sample labeling, hybridization, scanning, and data acquisition for Affymetrix GeneChip standard expression microarrays
- DNA sample hybridization, scanning, and data acquisition for Affymetrix GeneChip Mapping and Tiling microarrays.
- Analysis of RNA sample concentration, purity and quality
Other services
High-throughput liquid handling
- RCA DNA template preparation in a 96-well format
- Custom high throughput liquid handling utilizing our Beckman Coulter Biomek FX liquid handling robot
Robotic bacterial colony picking
- High-throughput bacterial colony picking and gridding utilizing our Genetix QPixII colony picking robot.
Instrumentation available for you to use
- Use our Axon GenePix microarray scanner to scan microarrays you have processed in your own lab.
- Utilize our Qiagen TissueLyser to prepare samples for microarray experiments.
- Assess RNA and DNA sample concentration and purity using our NanoDrop spectrophotometer.