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Protocols, procedures & presentations

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Commonly-used protocols in the University Park Cytometry Facility.

Sorting Quickstart
Thinking about sorting?? Here are a few details on how to prepare cells and estimate sort rates.
EMA procedure for detecting nonviable cells in a population to be fixed
A 2-page protocol available as a pdf file.
PFRET: spectral imaging FV1000 global instructions
A 3-page protocol available as a pdf file.
Virtual channel for simultaneous three-color confocal on FV300
A 1-page protocol available as a pdf file.
Fluorescence microscopy lecture: PDF
Download a pdf file of the full basic fluorescence microscopy lecture.
Fluorescence Microscopy lecture: Powerpoint
A powerpoint version of the general microscopy lecture.
Flow Cytometry lecture: Powerpoint
General flow cytometry lecture