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Olympus Fluoview 300
Although this basic confocal laser scanning microscope requires manual filter and laser set-ups, it is capable of highly sensitive and sophisticated multi-color imaging.
Overview
Blue, red and green lasers for multi-color imaging- X, Y, Z scanning for 3-D reconstruction
- After training, users are expected to image on their own
Cost
- $30 per hour
Details
- Microscope: Olympus IX70 inverted microscope with fluorescence burner
- Objectives: Uplan4X, UplanFL 10X/0.30, planFL 20X/0.50, UplanFL 40X/0.75, PlanApo 60X/1.4oil, Plan Apo 100X/1.3
- Lasers (excitation sources): Three single-line lasers with individual shutters that are software-controlled for sequential acquisition. Blue argon (488nm, 10mW), Green HeNe (543nm, 1mW), Red HeNe (633nm, 10mW)
- Scanning modes: 1-dimensional (spot-scan), 2-dimensional (X-Y vector, X-Z, or time), 3-dimensional (X-Y-Z, or X-Y-time) or 4 dimensional (X-Y-Z-time)
- Transmitted light: External halogen light source connected to microscope via fiber cables, external PMT for light collection. DIC optics are available for all objectives.
- Resolution: Confocal LSM improves the resolution of images by recording fluorescence or reflected light when a laser is focused at set foclal planes in tissues and cells, rejecting all other light coming from planes above or below the one of interest. This improves resolution of sub-micron structures within fixed and live cells and tissues. A series of images can be recorded axially and either analyzed separately or incorporated into a composite image.
- Software: Fluoview; captures files as MultiTiffs and displays as Tiff files
- Output: Files will be transferred to an off-line computer. Users will transfer data via FTP or download it to a writeable CD-ROM or zip disk. Display Tiff images can be printed directly using graphics software (such as Photoshop or PowerPoint). MultiTiff images can be reanalyzed using the Fluoview software or NIH Image (download NIH Image from the NIH website).