Sections
Personal tools
You are here: home Facilities Cytometry Facility, University Park Fluorescence microscopes Olympus BX60 (digital scope)
Document Actions

Olympus BX60 (digital scope)

This wide-field microscope snaps high-resolution digital pictures using conventional manual filter cubes or more versatile automated filter wheels. Multi-color pictures can be super-imposed.

Overview

  • This epifluorescent microscope includes a conventional FM with a software-controlled stepper motor to focus the illumination from a mercury vapor source at a series of pre-determined planes through cells producing a tile of images.
  • Large image fields can be tiled to produce high resolution images of large samples.

Cost

  • $20 per hour

Details

  • Illumination: Mercury vapor, provided through either rapid automated shutter or conventional filter cubes. Excitation wavelengths from UV to Red are available.
  • Objectives: UplanFL 10X/0.30, UplanFL 20X/0.50, UplanFL 40X/0.75, UplanFL 100X/1.3 oil. All objectives have DIC optics.
  • Digital camera: Hamamatsu cooled digital camera: ORCA 100 (Model C4742-95). Resolution of 1.3 million pixels and 8 or 12 bit digital output.
  • Controller: A Prior controller with joystick controls focus and location.
  • Software: Simple PCI from C-Imaging Systems.
  • Output: Files will be transferred to an off-line computer. Users will transfer data via FTP, or download it to a writeable CD-ROM or zip disk as Tiff files. Images can be printed using graphics software such as Photoshop or Powerpoint.