Cytopeia Influx

Overview

• Sorting rates of up to 30,000 particles per second
• Sterile sorts into tubes or multiwell dishes
• Option to add additional lasers and detectors
• Time can be used as a parameter (essential for detecting enzyme activity or calcium changes, for example)

Cost

• $60 per hour

Details

• Particle size sensitivity: resolved 0.5um particles from background
• Lasers: Three solid-state lasers: violet (408nm), blue (488nm) and red (635nm). Laser and detector cassettes with unique excitation and emissions. Detectors are not shared. Each laser passes through a pinhole with a unique time window. Crosstalk between lasers is virtually impossible.
• Signal amplification: Linear and log amplification with 4-decade log transformation, pulse width, peak height and peak area for each parameter, plus time
• Sample handling: Single-tube sampler accepts Falcon #352063 12 x 75 mm tubes
• Sample concentration: Single particle suspension of sample at optimal concentration of 10-20 x 10e6 cells per ml
• Sorting: The sorter has a through-put that averages 20,000 particles per second. With the standard setup, two populations are usually sorted into 12 x 75 tubes with media. The programmable micro-titer well tray configurations deposit single cells into 96 well or 384 well microtitre plates.
• Software: Sigot software controls the sorts. Listmode data files can be collected and reanalyzed in offline flow cytometry software packages such as FCS Express (available from us), or WinMDI (available free from http://facs.scripps.edu/recovery.html)