Document Actions
Coulter Elite ESP
A versatile cell sorter capable of using a wide variety of reagents and cell types. Sorting rates range from 2000-10,000 cells per second.
Overview
- Able to physically sort selected cell types.
- Can perform multi-laser experiments.
- Standard machine modifications include: a choice of flow tips with different size orifices for small or large particles, beam shaping optics to change the laser beam shape, and multi-laser choices.
- An autoclone attachment is available for sterile single cell cloning.
- Time can be used as a parameter.
Cost
- $55/hour
Details
- Particle size sensitivity: resolves 0.5um particles from background.
- Sensitivity: less than 1000 molecules of fluorescein equivalents.
- Signal amplification: Argon Ion air-cooled (15mW/488nm), helium-neon laser (15mW/633nm), water-cooled argon ion laser (200mW/UV).
- Sample handling: single tube sampler accepts 12 X 75 mm tubes.
- Sample concentration: single particle suspension of sample at optimal concentration of 50,000 to 5,000,000 per ml of suspending fluid.
- Sorting: the sorter has a through-put that averages 5,000 to 10,000 particles per second. With the standard setup two populations are sorted usually into 15 ml centrifuge tubes with media. The autoclone attachment allows sorting into multi-well dishes and is ideal for single-cell cloning.
- Software: Coulter elite software with a MS-DOS operating system. Expo 2 windows based software. Files can also be analyzed on the Windows-based Expo 2 multifile analysis program, available in the flow lab and a variety of other free-ware data analyses programs (e.g., see http://facs.scripps.edu/software.html)