Proteomics short course

Proteomics and Mass Spectrometry Core Facility is sponsoring a week-long Proteomics workshop aimed at introducing users to commonly used proteomics techniques and basic skills which can be incorporate into your research projects. 

Techniques covered

• 2D Gel Electrophoresis
• image analysis
• automated spot cutting
• in-gel digestion
• MALDI-TOF analysis
• 1D and 2D nanoLC-MS/MS analysis
• protein database searching

Enrollment

• Enrollment is limited to 6 people.
• If space is not available in this workshop, you may ask to be included in the waiting list for upcoming workshops which are planned to be held quarterly.

Fees

• The fee for the workshop is $500 per person
• payable by IDCC (credited to 430-46 1001J, send to user ID: qub8)

Questions / requests

• Please e-mail all requests to ms@huck.psu.edu

Schedule

• Following schedule is tentative.
• Workshops run from 9:00 AM to 4:00 PM daily.

Day 1 - Mon Aug 3, 2009

• Background Lecture
• Biological Mass Spectrometry
• Sample Preparation for 2D-GE

Day 2 - Tues Aug 4, 2009

• First dimension of 2D-GE, Isoelectric Focusing (IEF)
• Protein MW determination by LC-MS

Day 3 - Wed Aug 5, 2009

• Second dimension of 2D-GE, SDS-PAGE
• Gel staining
• Scanning with densitometer and laser scanner
• Image Analysis by PDQuest

Day 4 - Thurs Aug 6, 2009

• Automated spot cutting
• In-gel digestion

Day 5 - Fri Aug 7, 2009

• Peptide Mass Fingerprinting by MALDI-TOF
• Protein analysis by nanoLC-MS/MS and identification by protein database search